tailieunhanh - Báo cáo khoa học: "Genetic characterization of Barbari goats using microsatellite markers"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Genetic characterization of Barbari goats using microsatellite markers | J. Vet. Sci. 2009 10 1 73-76 DOI JOURNAL OF Veterinary Science Genetic characterization of Barbari goats using microsatellite markers J. Ramamoorthi K. Thilagam S. N. Sivaselvam S. M. K. Karthickeyan Department of Animal Genetics and Breeding Madras Veterinary College Tamilnadu Veterinary and Animal Sciences University Chennai-600 007 India Genetic variation in Barbari goats a highly prolific breed distributed widely in the northern part of India known for better milk and meat quality was studied as a part of genetic characterization and conservation. The genomic DNA from 50 unrelated Barbari goats were amplified via PCR with a panel of 21 microsatellite markers and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining. The number of alleles ranged from 4 to 11 with allele sizes ranging from 88 to 220 bp. The distribution of allele frequencies was between and . Polymorphism information content varied from to . The population was not in Hardy-Weinberg equilibrium for all except two microsatellite loci ILSTS044 and ILSTS060 . The observed heterozygosity ranged from to while the expected heterozygosity ranged from to . Based on the results of the present study there is a good scope for exploiting the genetic variability in the Barbari goats for further improvement of performance. Keywords Barbari goats genetic characterization heterozygosity microsatellite markers polymorphism Introduction Breed characterization requires basic knowledge of genetic variations that can be effectively measured within and between populations. The characterization data of Indian goats is imprecise because earlier studies based on morphological and biochemical markers did not present a true picture of their relationships. These markers have many limitations in identifying breed specificity as they do not have high resolving and distinguishing power among the closely