tailieunhanh - Báo cáo khoa học: "Experimental infection of chickens, ducks and quails with the highly pathogenic H5N1 avian influenza virus"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Experimental infection of chickens, ducks and quails with the highly pathogenic H5N1 avian influenza virus | J. Vet. Sci. 2009 10 1 53-60 DOI JOURNAL OF Veterinary Science Experimental infection of chickens ducks and quails with the highly pathogenic H5N1 avian influenza virus Ok-Mi Jeong Min-Chul Kim Min-Jeong Kim Hyun-Mi Kang Hye-Ryoung Kim Yong-Joo Kim Seong-Joon Joh Jun-Hun Kwon Youn-Jeong Lee National Veterinary Research and Quarantine Service Anyang 430-824 Korea Highly pathogenic avian influenza viruses HPAIV of the H5N1 subtype have spread since 2003 in poultry and wild birds in Asia Europe and Africa. In Korea the highly pathogenic H5N1 avian influenza outbreaks took place in 2003 2004 2006 2007 and 2008. As the 2006 2007 isolates differ phylogenetically from the 2003 2004 isolates we assessed the clinical responses of chickens ducks and quails to intranasal inoculation of the 2006 2007 index case virus A chicken Korea IS 06. All the chickens and quails died on 3 days and 3-6 days post-inoculation DPI respectively whilst the ducks only showed signs of mild depression. The uninoculated chickens and quails placed soon after with the inoculated flock died on and DPI respectively. Both oropharyngeal and cloacal swabs were taken for all three species during various time intervals after inoculation. It was found that oropharyngeal swabs showed higher viral titers than in cloacal swabs applicable to all three avian species. The chickens and quails shed the virus until they died up to 3 to 6 days after inoculation respectively whilst the ducks shed the virus on 2-4 DPI. The postmortem tissues collected from the chickens and quails on day 3 and days 4-5 and from clinically normal ducks that were euthanized on day 4 contained the virus. However the ducks had significantly lower viral titers than the chickens or quails. Thus the three avian species varied significantly in their clinical signs mortality tissue virus titers and duration of virus shedding. Our observations suggest that duck and quail farms should be monitored particularly .