tailieunhanh - LABORATORY STOCK SOLUTIONS AND EQUIPMENT: Common Stock Solutions, Buffers, and Media

Market concentration can be measured by looking at the share of market capitalization accounted for by the large stocks. These large stocks are seen as the leading 3 to 5 firms in the market (Maunder et al. 1991). In many economies only a few companies dominate the stock market (Bundoo 1999). High concentration is not desirable as it can adversely affect liquidity, as it is common to find a negative correlation between concentration and liquidity. Table 5 shows the market concentration percentages. The leading 5 companies are considered to be large stocks in Fiji and the selection of these. | LABORATORY STOCK SOLUTIONS AND APPENDIX 2 EQUIPMENT Common Stock Solutions Buffers and Media APPENDIX 2A RECIPES This section describes the preparation of buffers and reagents used in this manual for cell culture manipulation of tissue and cell biological methods. When preparing solutions use deionized or distilled water and reagents ofthe highest available grade. Sterilization by filtration through a filter or by autoclaving is recommended for most solutions stored at room temperature and is essential for cell culture applications. Where storage conditions are not specified store up to 6 months at room temperature. Discard any reagent that shows evidence of contamination precipitation or discoloration. Acid concentrated stock solutions See Table . Acid precipitation solution 1 M Hc1 M sodium pyrophosphate Nucleic acids can also be precipitated with a 10 w v solution of trichloroacetic acid TCA however this recipe is cheaper easier to prepare and just as efficacious. Ammonium hydroxide concentrated stock solution See Table . Ammonium acetate 10 M Dissolve g ammonium acetate in 150 ml H2O Add H2O to 500 ml Ammonium sulfate saturated 76 g ammonium sulfate 100 ml H2O Heat with stirring to just below boiling point Let stand overnight at room temperature Table Molarities and Specific Gravities of Concentrated Acids and Basesa Acid base Molecular weight by weight Molarity approx. Specific gravity 1 M solution ml liter Acetic acid glacial Ammonium hydroxide 28 Formic acid 90 98 Hydrochloric acid 36 Nitric acid 70 Perchloric acid 60 72 Phosphoric acid 85 Sulfuric acid 98 aCAUTION Handle strong acids and bases carefully. Laboratory Stock Solutions and Equipment Current Protocols in Cell Biology 1998 Copyright 1998 by John

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