tailieunhanh - Báo cáo khoa học: "Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium | J. Vet. Sci. 2009 10 4 299-304 DOI JOURNAL OF Veterinary Science Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium 1 2 1 3 3 So Yeong Lee Kyong-Ah Yoon Soo Hwa Jang Erdene Ochir Ganbold Dembereldorj Uuriintuya Sang-Mo Shin4 Pan Dong Ryu1 Sang-Woo Joo3 laboratory of Pharmacology College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 2Research Institute and Hospital National Cancer Center Goyang 410-769 Korea 3Department of Chemistry Soongsil University Seoul 156-743 Korea 4Department of Mechatronics Gwangju Institute of Science and Technology Gwangju 500-712 Korea The vibrational spectral differences of normal and lung cancer cells were studied for the development of effective cancer cell screening by means of attenuated total reflection infrared spectroscopy. The phosphate monoester symmetric stretching Vs POj2- band intensity at 970 cm-1 and the phosphodiester symmetric stretching Vs PO2- band intensity at 1 085 cm-1 in nucleic acids and phospholipids appeared to be significantly strengthened in lung cancer cells with respect to the other vibrational bands compared to normal cells. This finding suggests that more extensive phosphorylation occur in cancer cells. These results demonstrate that lung cancer cells may be prescreened using infrared spectroscopy tools. Keywords cancer cell screening cell monitoring infrared spectroscopy lung cancers phosphorylation Introduction Cancer is the principal causes of death worldwide according to the World Health Organization with lung cancer being one of the most commonly diagnosed types of cancer in men and the fourth most common cause of cancer-related death in men 18 . The early detection of invasive lung cancer is essential in reducing its mortality rate. It has been problematic however to determine the quantitative differences in spectral signatures between normal precancerous and cancer cells without causing structural and .