tailieunhanh - Báo cáo khoa học: "Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs | J. Vet. Sci. 2008 9 4 387-393 JOURNAL OF Veterinary Science Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs Byung-Jun Jang1 Ye-Eun Byeon1 Ji-Hey Lim1 Hak-Hyun Ryu1 Wan Hee Kim1 Yoshihisa Koyama3 Masanori Kikuchi4 Kyung-Sun Kang2 Oh-Kyeong Kweon1 Department of Veterinary Surgery 2Laboratories of Stem Cell and Tumor Biology Department of Veterinary Public Health College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 3Department of Biodesign Institute of Biomaterials Bioengineering Tokyo Medical Dental University Tokyo Japan 4Biomaterials Center National Institute for Materials Science Tsukuba Japan This study was performed to evaluate the osteogenic effect of allogenic canine umbilical cord blood-derived mesenchymal stem cells UCB-MSCs mixed with beta-tricalcium phosphate P-TCP in orthotopic implantation. Seven hundred milligrams of P -TCP mixed with 1 X 106 UCB-MSCs diluted with ml of saline group CM and mixed with the same volume of saline as control group C were implanted into a cm diaphyseal defect and wrapped with PLGC membrane in the radius of Beagle dogs. Radiographs of the antebrachium were made after surgery. The implants were harvested 12 weeks after implantation and specimens were stained with H E toluidine blue and Villanueva-Goldner stains for histological examination and histomorphometric analysis of new bone formation. Additionally UCB-MSCs were applied to a dog with non-union fracture. Radiographically continuity between implant and host bone was evident at only one of six interfaces in group C by 12 weeks but in three of six interfaces in group CM. Radiolucency was found only near the bone end in group C at 12 weeks after implantation but in the entire graft in group CM. Histologically bone formation was observed around P-TCP in longitudinal sections of implant in both groups. Histomorphometric analysis .