tailieunhanh - Báo cáo khoa học: "Immunohistochemical study of galectin-3 in mature and immature bull testis and epididymis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical study of galectin-3 in mature and immature bull testis and epididymis | J. Vet. Sci. 2008 9 4 339-344 JOURNAL OF Veterinary Science Immunohistochemical study of galectin-3 in mature and immature bull testis and epididymis 1 3 1 4 5 1 2 1 2 Hwanglyong Kim Meejung Ahn Changjong Moon Seungjoon Kim Youngheun Jem Hong-Gu Joo Taekyun Shin1 2 College of Veterinary Medicine 2Applied Radiological Science Research Institute Cheju National University Jeju 690-756 Korea 3Veterinary Laboratory Jeju Special Self-Governing Province Jeju 690-962 Korea 4Department of Veterinary Anatomy College of Veterinary Medicine Chonnam National University Gwangju 500-757 Korea 5Department of Veterinary Obstetrics College of Veterinary Medicine Kyungpook National University Daegu 702-701 Korea Galectin-3 a member of the p -galactoside-binding protein family has been implicated in mammalian sperm maturation. We examined galectin-3 expression in the testis and epididymis of sexually mature and immature bulls. Western blot analysis showed varying levels of galectin-3 in the bull testis and epididymis and galectin-3 immunoreactivity was higher in the mature testis and epididymis than in immature organs. Galectin-3 was primarily localized in interstitial cells of the immature bull testis and in the peritubular myoid and interstitial cells of the mature testis. In the immature epididymis head galectin-3 was primarily in the principal and basal cells of the epithelium. In the mature epididymis head moderate levels of galectin-3 were detected in the sperm while low levels were found in the stereocilia epithelium and connective tissue. In the immature epididymis body moderate protein levels were detected in the principal cells while lower levels were found in the basal cells. The mature epididymis body showed moderate levels of galectin-3 immunostaining in the stereocilia and epithelium but low levels in the connective tissue. In the immature epididymis tail only low levels of galectin-3 staining were found in the epithelium whereas the mature epididymis tail showed high .