tailieunhanh - Báo cáo khoa học: "Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus)"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer (Cervus nippon laiouanus) | J. Vet. Set. 2007 8 3 299-301 JOURNAL OF Short Communication Veterinary Science Identification of single-nucleotide polymorphisms of the prion protein gene in sika deer Cervus nippon laiouanus Jeong Joong-Bok Lee Seung-Yong Park Chang-Seon Song Bo-Sook Kim Jung-Rae Rho Mi-Hyun Yoo2 Byung-Hoon Jeong3 Yong-Sun Kim3 In-Soo Choi1 1 Department of Infectious Diseases College of Veterinary Medicine Konkuk University Seoul 143-701 Korea 2Animal Research Division Seoul Grand Park Zoo Gwacheon 427-420 Korea 3Ilsong Institute of Life Science Hallym University Anyang 431-060 Korea Polymorphisms of the prion protein gene PRNP have been detected in several cervid species. In order to confirm the genetic variations this study examined the DNA sequences of the PRNP obtained from 33 captive sika deer Cervas nippon laiouanus in Korea. A total of three single-nucleotide polymorphisms SNPs at codons too 136 and 226 in the PRNP of the sika deer were identified. The polymorphic site located at codon 100 has not been reported. The SNPs detected at codons 100 and 226 induced amino acid substitutions. The SNP at codon 136 was a silent mutation that does not induce any amino acid change. The genotype and allele frequencies were determined for each of the SNPs. Key words PRNR sika deer single-nucleotide polymorphism The susceptibility to prion diseases in humans and sheep is strongly associated with different genotypes of the prion protein gene PRNP 1 2 Chronic wasting disease CWD which is a form of transmissible spongiform encephalopathy principally affects mule deer white-tailed deer and Rocky Mountain elk 9 . The specific PRNP allele patterns found in these species significantly influence their susceptibility to CWD as well as the progression of the disease. Although the PRNP of the sika deer encodes almost identical amino acids to those of CWD-susceptible deer species there is still no definitive report on CWD-infected sika deer. Therefore we are currently unable to .