tailieunhanh - Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury | J. Vet. Sci. 2009 10 4 273-284 DOI JOURNAL OF Veterinary Science Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury 1 1 1 2 2 3 Hak-Hyun Ryu Ji-Hey Lim Ye-Eun Byeon Jeong-Ran Park Min-Soo Seo Young-Won Lee Wan Hee Kim1 Kyung-Sun Kang2 Oh-Kyeong Kweon1 Department of Veterinary Surgery and 2Laboratory of Stem Cell and Tumor Biology Department of Veterinary Public Health College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 3College of Veterinary Medicine Research Institute of Veterinary Medicine Chungnam National University Daejeon 305-764 Korea In this study we evaluated if the implantation of allogenic adipose-derived stem cells ASCs improved neurological function in a canine spinal cord injury model. Eleven adult dogs were assigned to three groups according to treatment after spinal cord injury by epidural balloon compression C group no ASCs treatment as control V group vehicle treatment with PBS and ASC group ASCs treatment . ASCs or vehicle were injected directly into the injured site 1 week after spinal cord injury. Pelvic limb function after transplantation was evaluated by Olby score. Magnetic resonance imaging somatosensory evoked potential SEP histopathologic and immunohistichemical examinations were also performed. Olby scores in the ASC group increased from 2 weeks after transplantation and were significantly higher than C and V groups until 8 weeks p . However there were no significant differences between the C and V groups. Nerve conduction velocity based on SEP was significantly improved in the ASC group compared to C and V groups p . Positive areas for Luxol fast blue staining were located at the injured site in the ASC group. Also GFAP Tuj-1 and NF160 were observed immunohistochemically in cells derived from implanted ASCs. These results suggested that improvement in neurological function .