tailieunhanh - Báo cáo khoa học: "Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs | J. Vet. Set. 2007 8 3 275-282 JOURNAL OF Veterinary Science Transplantation of canine umbilical cord blood-derived mesenchymal stem cells in experimentally induced spinal cord injured dogs Ji-Hey Lim1 Ye-Eun Byeon1 Hak-Hyun Ryu1 Yun-Hyeok Jeong2 Young-Won Lee3 Wan Hee Kim1 Kyung-Sun Kang2 Oh-Kyeong Kweon1 1 Department of Veterinary Surgery 2LaboratoryofStem Cell and Tumor Biology Department of Veterinary Public Health College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 3 College of Veterinary Medicine Research Institute of Veterinary Medicine Chungnam National University Daejeon 305-764 Korea This study was to determine the effects of allogenic umbilical cord blood UCB -derived mesenchymal stem cells MSCs and recombinant methionyl human granulocyte colony-stimulating factor rmhGCSF on a canine spinal cord injury model after balloon compression at the first lumbar vertebra. Twenty-five adult mongrel dogs were assigned to five groups according to treatment after a spinal cord injury no treatment CN saline treatment CP rmhGCSF treatment G UCB-MSCs treatment UCB MSC co-treatment UCBG . The UCB-MSCs isolated from cord blood of canine fetuses were prepared as 106 cells 150 pl saline. The UCB-MSCs were directly injected into the injured site of the spinal cord and rmhGCSF was administered subcutaneously 1 week after the induction of spinal cord injury. The Olby score magnetic resonance imaging somatosensory evoked potentials and histopathological examinations were used to evaluate the functional recovery after transplantation. The Olby scores of all groups were zero at the 0-week evaluation. At 2 week after the transplantation the Olby scores in the groups with the UCB-MSC and UCBG were significantly higher than in the CN and CP groups. However there were no significant differences between the UCB-MSC and UCBG groups and between the CN and CP groups. These comparisons remained stable at 4 and 8 week after transplantation. There was significant .