tailieunhanh - Ruta 6 selectively induces cell death in brain cancer cells but proliferation in normal peripheral blood lymphocytes: A novel treatment for human brain cancer

Although the psychosocial oncology literature is strong, substantial limitations and unanswered questions remain. Much of the research has focused on heterogeneous samples of patients who vary widely across disease site (., melanoma vs. breast cancer), stage of progression (., localized vs. metastatic), and phase of illness (., initial diagnosis vs. long-term survival). The challenges patients face vary widely across these dimensions, and therefore, it is crucial for research designs to take this into consideration | INTERNATIONAL JOURNAL OF ONCOLOGY 23 975-982 2003 Ruta 6 selectively induces cell death in brain cancer cells but proliferation in normal peripheral blood lymphocytes A novel treatment for human brain cancer SEN PATHAK1 2 ASHA S. MULTANI1 PRATIP BANERJI3 and PRASANTA BANERJI3 Departments of 1Cancer Biology and 2Laboratory Medicine The University of Texas . Anderson Cancer Center Houston TX 77030 USA 3PBH Research Foundation 10 3 1 Elgin Road Kolkata 700 020 West Bengal India Received April 16 2003 Accepted May 28 2003 Abstract. Although conventional chemotherapies are used to treat patients with malignancies damage to normal cells is problematic. Blood-forming bone marrow cells are the most adversely affected. It is therefore necessary to find alternative agents that can kill cancer cells but have minimal effects on normal cells. We investigated the brain cancer cell-killing activity of a homeopathic medicine Ruta isolated from a plant Ruta graveolens. We treated human brain cancer and HL-60 leukemia cells normal B-lymphoid cells and murine melanoma cells in vitro with different concentrations of Ruta in combination with Ca3 PO4 2. Fifteen patients diagnosed with intracranial tumors were treated with Ruta 6 and Ca3 PO4 2. Of these 15 patients 6 of the 7 glioma patients showed complete regression of tumors. Normal human blood lymphocytes B-lymphoid cells and brain cancer cells treated with Ruta in vitro were examined for telomere dynamics mitotic catastrophe and apoptosis to understand the possible mechanism of cell-killing using conventional and molecular cytogenetic techniques. Both in vivo and in vitro results showed induction of survival-signaling pathways in normal lymphocytes and induction of death-signaling pathways in brain cancer cells. Cancer cell death was initiated by telomere erosion and completed through mitotic catastrophe events. We propose that Ruta in combination with Ca3 PO4 2 could be used for effective treatment of brain cancers particularly