tailieunhanh - Báo cáo y học: "Experimental annotation of the human pathogen Candida albicans coding and noncoding transcribed regions using high-resolution tiling arrays"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Experimental annotation of the human pathogen Candida albicans coding and noncoding transcribed regions using high-resolution tiling arrays. | Sellam et al. Genome Biology 2010 11 R71 http 2010 11 7 R71 w Genome Biology RESEARCH Open Access Experimental annotation of the human pathogen Candida albicans coding and noneoding transcribed regions using high-resolution tiling arrays Adnane Sellam 1 2 Hervé Hogues1 Christopher Askew1 3 Faiza Tebbji1 3 Marco van het Hoog1 Hugo Lavoie4 Carol A Kumamoto5 Malcolm Whiteway1 3 and André Nantel 1 2 Abstract Background Compared to other model organisms and despite the clinical relevance of the pathogenic yeast Candida albicans no comprehensive analysis has been done to provide experimental support of its in silico-based genome annotation. Results We have undertaken a genome-wide experimental annotation to accurately uncover the transcriptional landscape of the pathogenic yeast C. albicans using strand-specific high-density tiling arrays. RNAs were purified from cells growing under conditions relevant to C. albicans pathogenicity including biofilm lab-grown yeast and serum-induced hyphae as well as cells isolated from the mouse caecum. This work provides a genome-wide experimental validation for a large number of predicted ORFs for which transcription had not been detected by other approaches. Additionally we identified more than 2 000 novel transcriptional segments including new ORFs and exons noncoding RNAs ncRNAs as well as convincing cases of antisense gene transcription. We also characterized the 5 and 3 UTRs of expressed ORFs and established that genes with long 5 UTRs are significantly enriched in regulatory functions controlling filamentous growth. Furthermore we found that genomic regions adjacent to telomeres harbor a cluster of expressed ncRNAs. To validate and confirm new ncRNA candidates we adapted an iterative strategy combining both genome-wide occupancy of the different subunits of RNA polymerases I II and III and expression data. This comprehensive approach allowed the identification of different families of ncRNAs. Conclusions In .