tailieunhanh - Bioluminescence Recent Advances in Oceanic Measurements and Laboratory Applications Part 14

Tham khảo tài liệu 'bioluminescence recent advances in oceanic measurements and laboratory applications part 14', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | 186 Bioluminescence - Recent Advances in Oceanic Measurements and Laboratory Applications 590000 in 585000 c q 580000 o o 575000 o g 570000 c 565000 E. 560000 1 555000 JO 2 550000 0 20 40 60 80 100 time I s Oil was used to prevent the bacterial environment from drying. Glass with an amino group modification MAS coated glass slides Matsunami Glass Ind. Ltd. was used for the adsorption. The glass was soaked in a marine-broth-based bacterial suspension overnight. A measurement was performed using a luminescence meter GENE LIGHT GL-200S Microtec Nichion . Fig. 16. Effect of irradiation to the luminescence from cells adsorbed on a glass surface. 4. Conclusion Oscillation in the bacterial bioluminescence mode is strongly dependent on the amount of oxygen supply to the solution. There is no clear relationship between the DO concentration and luminescence intensity perhaps due to the consumption of oxygen by both the luminescence and respiration. The oscillation occurred at a very low DO concentration and when the time course of cell density was plotted with the same timescale as the luminescence intensity the cell growth rate seemed to decrease after the strong luminescence. The fluorescence ability of LumP seemed constant during the oscillation period but at the beginning and at the end it seemed to decrease. The characterisation of luminescence from a smaller number of cells would be necessary for further investigation of oscillation considering that the suspension is a mixture of cell groups with a variety of cell phases. Oscillation in Bacterial Bioluminescence 187 5. Acknowledgments The author thanks Dr. Hajime Karatani of the Kyoto Institute of Technology for his participation in discussions and Shoji Yamada Kenshin Tamura Shingo Kuriyama Mika Mochizuki and Hajime Kimoto for their assistance with the experiments. 6. References Aivasidis A. Melidis P. Georgiou D. 2002 . Use of a microbial sensor a new approach to the measurement of inhibitory effects on the microbial