tailieunhanh - Báo cáo y học: "The developmental expression dynamics of Drosophila melanogaster transcription factors"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: The developmental expression dynamics of Drosophila melanogaster transcription factors. | Adryan and Teichmann Genome Biology 2010 11 R40 http 2010 11 4 R40 w Genome Biology RESEARCH _ Open Access The developmental expression dynamics of Drosophila melanogaster transcription factors Boris Adryan 1 2 and Sarah ATeichmann 1 Abstract Background Site-specific transcription factors TFs are coordinators of developmental and physiological gene expression programs. Their binding to cis-regulatory modules of target genes mediates the precise cell- and contextspecific activation and repression of genes. The expression of TFs should therefore reflect the core expression program of each cell. Results We studied the expression dynamics of about 750 TFs using the available genomics resources in Drosophila melanogaster. We find that 95 of these TFs are expressed at some point during embryonic development with a peak roughly between 10 and 12 hours after egg laying the core stages of organogenesis. We address the differential utilization of DNA-binding domains in different developmental programs systematically in a spatio-temporal context and show that the zinc finger class of TFs is predominantly early expressed while Homeobox TFs exhibit later expression in embryogenesis. Conclusions Previous work dissecting cis-regulatory modules during Drosophila development suggests that TFs are deployed in groups acting in a cooperative manner. In contrast we find that there is rapid exchange of co-expressed partners amongst the fly TFs at rates similar to the genome-wide dynamics of co-expression clusters. This suggests there may also be a high level of combinatorial complexity of TFs at cis-regulatory modules. Background In the post-genomic era transcriptional regulatory relationships are often displayed in the form of a gene regulatory network GRN in which the nodes represent genes and the edges are the regulatory interaction of activation or repression . Developmental geneticists turned to network biology decades ago because the complex relationships between