tailieunhanh - Báo cáo y học: "Genomic characterization of the Yersinia genus"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Genomic characterization of the Yersinia genus. | Chen et al. Genome Biology 2010 11 R1 http 2010 11 1 R1 Genome Biology RESEARCH Open Access Genomie characterization of the Yersinia genus 11 11 11 27 2 2 Peter E Chen Christopher Cook Andrew C Stewart Niranjan Nagarajan Dan D Sommer Mihai Pop 1 111 1 Brendan Thomason Maureen P Kiley Thomason Shannon Lentz Nichole Nolan Shanmuga Sozhamannan Alexander Sulakvelidze3 Alfred Mateczun 1 Lei Du4 Michael E Zwick1 5 Timothy D Read1 5 6 Abstract Background New DNA sequencing technologies have enabled detailed comparative genomic analyses of entire genera of bacterial pathogens. Prior to this study three species of the enterobacterial genus Yersinia that cause invasive human diseases Yersinia pestis Yersinia pseudotuberculosis and Yersinia enterocolitica had been sequenced. However there were no genomic data on the Yersinia species with more limited virulence potential frequently found in soil and water environments. Results We used high-throughput sequencing-by-synthesis instruments to obtain 25- to 42-fold average redundancy whole-genome shotgun data from the type strains of eight species Y. aldovae Y. bercovieri Y. frederiksenii Y. kristensenii Y. intermedia Y. mollaretii Y. rohdei and Y. ruckeri. The deepest branching species in the genus Y. ruckeri causative agent of red mouth disease in fish has the smallest genome Mb although it shares the same core set of approximately 2 500 genes as the other members of the species whose genomes range in size from to Mb. Yersinia genomes had a similar global partition of protein functions as measured by the distribution of Cluster of Orthologous Groups families. Genome to genome variation in islands with genes encoding functions such as ureases hydrogeneases and B-12 cofactor metabolite reactions may reflect adaptations to colonizing specific host habitats. Conclusions Rapid high-quality draft sequencing was used successfully to compare pathogenic and non-pathogenic members of the Yersinia genus. This .