tailieunhanh - Báo cáo y học: "Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea, and their metagenomes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea, and their metagenomes. | Weinberg et al. Genome Biology 2010 11 R31 http 2010 11 3 R31 Genome Biology RESEARCH Open Access Comparative genomics reveals 104 candidate structured RNAs from bacteria archaea and their metagenomes 1 2 1 Zasha Weinberg 1 Joy X Wang Jarrod Bogue Jingying Yang Keith Corbino Ryan H Moy Ronald R Breaker1 2 3 Abstract Background Structured noncoding RNAs perform many functions that are essential for protein synthesis RNA processing and gene regulation. Structured RNAs can be detected by comparative genomics in which homologous sequences are identified and inspected for mutations that conserve RNA secondary structure. Results By applying a comparative genomics-based approach to genome and metagenome sequences from bacteria and archaea we identified 104 candidate structured RNAs and inferred putative functions for many of these. Twelve candidate metabolite-binding RNAs were identified three of which were validated including one reported herein that binds the coenzyme S-adenosylmethionine. Newly identified cis-regulatory RNAs are implicated in photosynthesis or nitrogen regulation in cyanobacteria purine and one-carbon metabolism stomach infection by Helicobacter and many other physiological processes. A candidate riboswitch termed crcB is represented in both bacteria and archaea. Another RNA motif may control gene expression from 3 -untranslated regions of mRNAs which is unusual for bacteria. Many noncoding RNAs that likely act in trans are also revealed and several of the noncoding RNA candidates are found mostly or exclusively in metagenome DNA sequences. Conclusions This work greatly expands the variety of highly structured noncoding RNAs known to exist in bacteria and archaea and provides a starting point for biochemical and genetic studies needed to validate their biologic functions. Given the sustained rate of RNA discovery over several similar projects we expect that far more structured RNAs remain to be discovered from bacterial .