tailieunhanh - Báo cáo y học: "Catalogues of mammalian long noncoding RNAs: modest conservation and incompleteness"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Catalogues of mammalian long noncoding RNAs: modest conservation and incompleteness. | Open Access Research Catalogues of mammalian long noncoding RNAs modest conservation and incompleteness Ana C Marques and Chris P Ponting Address MRC Functional Genomics Unit University of Oxford Department of Physiology Anatomy and Genetics Oxford OX1 3QX UK. Correspondence Ana C Marques. Email Published 6 November 2009 Genome Biology 2009 10 R124 doi gb-2009-l0-ll-rl 24 The electronic version of this article is the complete one and can be found online at http 2009 10 11 R124 Received 8 August 2009 Revised 2l October 2009 Accepted 6 November 2009 2009 Marques et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background Despite increasing interest in the noncoding fraction of transcriptomes the number species-conservation and functions if any of many non-protein-coding transcripts remain to be discovered. Two extensive long intergenic noncoding RNA ncRNA transcript catalogues are now available for mouse over 3 000 macroRNAs identified by cDNA sequencing and 1 600 long intergenic noncoding RNA lincRNA intervals that are predicted from chromatin-state maps. Previously we showed that macroRNAs tend to be more highly conserved than putatively neutral sequence although only 5 of bases are predicted as constrained. By contrast over a thousand lincRNAs were reported as being highly conserved. This apparent difference may account for the surprisingly small fraction 11 of transcripts that are represented in both catalogues. Here we sought to resolve the reported discrepancy between the evolutionary rates for these two sets. Results Our analyses reveal lincRNA and macroRNA exon sequences to be subject to the same relatively low degree of sequence constraint. .