tailieunhanh - Báo cáo y học: " Regulatory interdependence of myeloid transcription factors revealed by Matrix RNAi analysis"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Regulatory interdependence of myeloid transcription factors revealed by Matrix RNAi analysis. | Open Access Regulatory interdependence of myeloid transcription factors revealed by Matrix RNAi analysis Yasuhiro Tomaru Christophe Simon Alistair RR Forrest Hisashi Miura Atsutaka Kubosaki Yoshihide Hayashizaki and Masanori Suzuki Addresses RIKEN Omics Science Center RIKEN Yokohama Institute 1-7-22 Suehiro-cho Tsurumi-ku Yokohama Kanagawa 230-0045 Japan. international Graduate School of Arts and Sciences Yokohama City University 1-7-29 Suehiro-Cho Tsurumi-Ku Yokohama 2300045 Japan. The Eskitis Institute for Cell and Molecular Therapies Griffith University Brisbane Innovation Park Don Young Road Nathan QLD 4111 Australia. Correspondence Masanori Suzuki. Email msuzuki@ Published 2 November 2009 Received 7 July 2009 Genome Biology 2009 10 R121 doi gb-2009-10-11-r121 Accepted 2 November 2009 The electronic version of this article is the complete one and can be found online at http 2009 10 11 R121 2009 Tomaru et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background With the move towards systems biology we need sensitive and reliable ways to determine the relationships between transcription factors and their target genes. In this paper we analyze the regulatory relationships between 78 myeloid transcription factors and their coding genes by using the matrix RNAi system in which a set of transcription factor genes are individually knocked down and the resultant expression perturbation is quantified. Results Using small interfering RNAs we knocked down the 78 transcription factor genes in monocytic THP-1 cells and monitored the perturbation of the expression of the same 78 transcription factors and 13 other transcription factor genes as well as 5 non-transcription factor .

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