tailieunhanh - Herbicides Environmental Impact Studies and Management Approaches Part 13

Tham khảo tài liệu 'herbicides environmental impact studies and management approaches part 13', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Genetic Diversity in Weeds 229 esterase with the slowest migration was named as Est-8 Figure 3 . These results confirmed a previous hypothesis that PAGE may be a powerful procedure for analysis of a P-esterases isozymes from leaf tissues of wild poinsettia plants. Eight loci for isoesterases were simultaneously and clearly evident in the same electrophoresis that is using only one enzymatic system. Isozyme studies in other Euphorbia species have revealed only 11 loci from analysis of eight enzymatic systems Park 2004 . The analysis of different enzymatic systems generally requires higher cost and time investments. Thus a- and P-esterase isozymes analysis in PAGE system may be used in further studies to detect genetic diversity in other Euphorbia species Fig. 2. Localities where seeds of wild poinsettia were collected at Mato Grosso Brazil MT and Parana Brazil PR states P1 and P11 Maringá PR P2 São Miguel do Iguaẹu PR P3 Terra Rica PR P4 and P9 Ivaiporã PR P5 Campo Verde MT P6 Ivatuba PR P7 Floral PR P8 Marialva PR P10 and P12 Floresta PR populations. Source Frigo et al. 2009 . 230 Herbicides - Environmental Impact Studies and Management Approaches High and low genetic diversity levels have been reported in different populations of wild poinsettia by DNA fragment analysis as molecular markers Vasconcelos et al. 2000 Winkler et al. 2003 . Deploying a- and P-esterase polymorphisms in the PAGE system Frigo et al. 2009 indicates that genetic diversity of wild poinsettia has higher mean values for grades of genetic variation number of alleles per locus proportion of polymorphic loci observed and expected proportion of heterozygous loci when compared to other Euphorbia species Park 2004 . The genetic variation in wild poinsettia is nearly as high as the genetic variability in Euphorbia ebracteolata a widespread species Park et al. 1999 . The proportion of polymorphic loci in 12 wild poinsettia populations is much higher than the mean proportion value 31 reported for .