tailieunhanh - Báo cáo y học: " Strand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across yeast specie"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Strand-specific RNA sequencing reveals extensive regulated long antisense transcripts that are conserved across yeast species. | Yassour et al. Genome Biology 2010 11 R87 http 2010 11 8 R87 Genome Biology RESEARCH Open Access Strand-speeifie RNA sequencing reveals extensive regulated long antisense transcripts that are eonserved aeross yeast speeies 1t 1t w I- 1 1 1 Moran Yassour Jenna Pfiffner Joshua Z Levin Xian Adiconis Andreas Gnirke Chad Nusbaum Dawn-Anne Thompson1 Nir Friedman3 4 Aviv Regev1 2 Abstract Background Recent studies in budding yeast have shown that antisense transcription occurs at many loci. However the functional role of antisense transcripts has been demonstrated only in a few cases and it has been suggested that most antisense transcripts may result from promiscuous bi-directional transcription in a dense genome. Results Here we use strand-specific RNA sequencing to study anti-sense transcription in Saccharomyces cerevisiae. We detect 1 103 putative antisense transcripts expressed in mid-log phase growth ranging from 39 short transcripts covering only the 3 UTR of sense genes to 145 long transcripts covering the entire sense open reading frame. Many of these antisense transcripts overlap sense genes that are repressed in mid-log phase and are important in stationary phase stress response or meiosis. We validate the differential regulation of 67 antisense transcripts and their sense targets in relevant conditions including nutrient limitation and environmental stresses. Moreover we show that several antisense transcripts and in some cases their differential expression have been conserved across five species of yeast spanning 150 million years of evolution. Divergence in the regulation of antisense transcripts to two respiratory genes coincides with the evolution of respiro-fermentation. Conclusions Our work provides support for a global and conserved role for antisense transcription in yeast gene regulation. Background Antisense transcription plays an important role in gene regulation from bacteria to humans. While the role of antisense .