tailieunhanh - Báo cáo y học: "MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays. | Open Access MethMarker user-friendly design and optimization of gene-specific DNA methylation assays Peter Schuffler Thomas Mikeska Andreas Waha Thomas Lengauer and Christoph Bock Addresses Max-Planck-Institut fur Informatik Campus 66123 Saarbrucken Germany. Molecular Pathology Research and Development Laboratory Department of Pathology Peter MacCallum Cancer Centre A Beckett Street Melbourne Victoria 8006 Australia. Department of Neuropathology University of Bonn Medical Centre Sigmund-Freud-Strabe 53105 Bonn Germany. Correspondence Christoph Bock. Email cbock@ Published 5 October 2009 Genome Biology 2009 10 RI05 doi gb-2009-I0-I0-rI 05 The electronic version of this article is the complete one and can be found online at http 2009 10 10 R105 Received 23 March 2009 Revised 19 August 2009 Accepted 5 October 2009 2009 Schuffler et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract DNA methylation is a key mechanism of epigenetic regulation that is frequently altered in diseases such as cancer. To confirm the biological or clinical relevance of such changes gene-specific DNA methylation changes need to be validated in multiple samples. We have developed the MethMarker http software to help design robust and cost-efficient DNA methylation assays for six widely used methods. Furthermore MethMarker implements a bioinformatic workflow for transforming disease-specific differentially methylated genomic regions into robust clinical biomarkers. Rationale Aberrant DNA methylation is a common event in many cancers 1 2 . Functionally cancer-specific hypermethylation imposes condensed chromatin structure upon CpG islands that normally exhibit an open and .