tailieunhanh - Báo cáo y học: " Many LINE1 elements contribute to the transcriptome of human somatic cells"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Many LINE1 elements contribute to the transcriptome of human somatic cells. | Open Access Researc h Many LINE1 elements contribute to the transcriptome of human somatic cells Sanjida H Rangwala Lili Zhang and Haig H Kazazian Jr Address Department of Genetics University of Pennsylvania School of Medicine Hamilton Walk Philadelphia Pennsylvania 19104 USA. Correspondence Haig H Kazazian. Email kazazian@ Received 20 May 2009 Revised 21 August 2009 Accepted 22 September 2009 Published 22 September 2009 Genome Biology 2009 10 RI00 doi gb-2009-10-9-r100 The electronic version of this article is the complete one and can be found online at http 2009 10 9 R100 2009 Rangwala et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background While LINE1 L1 retroelements comprise nearly 20 of the human genome the majority are thought to have been rendered transcriptionally inactive due to either mutation or epigenetic suppression. How many L1 elements escape these forms of repression and contribute to the transcriptome of human somatic cells We have cloned out expressed sequence tags corresponding to the 5 and 3 flanks of L1 elements in order to characterize the population of elements that are being actively transcribed. We also examined expression of a select number of elements in different individuals. Results We isolated expressed sequence tags from human lymphoblastoid cell lines corresponding to 692 distinct L1 element sites including 410 full-length elements. Four of the expression tagged sites corresponding to full-length elements from the human specific L1Hs subfamily were examined in European-American individuals and found to be differentially expressed in different family members. Conclusions A large number of different L1 element sites are expressed .