tailieunhanh - Báo cáo y học: " De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: De novo genome sequence assembly of a filamentous fungus using Sanger, 454 and Illumina sequence data. | Open Access De novo genome sequence assembly of a filamentous fungus using Sanger 454 and Illumina sequence data Scott DiGuistiniH Nancy YLiaoH Darren Platt Gordon Robertson Michael Seidel Simon K Chan T Roderick Docking Inanc Birol Robert A Holt Martin Hirst Elaine Mardis Marco A Marra Richard C Hamelin Jorg Bohlmann Colette Breuil and Steven JM Jones Addresses Department of Wood Science University of British Columbia Vancouver BC V6T 1Z4 Canada. BC Cancer Agency Genome Sciences Centre Vancouver BC V5Z 4E6 Canada. Amyris Biotechnologies Inc. Hollis Street Emeryville CA 94608 USA. Washington University School of Medicine Forest Park Ave St Louis MO 63108 USA. Natural Resources Canada rue du PEPS Ste-Foy Quebec G1V 4C7 Canada. Michael Smith Laboratories University of British Columbia Vancouver BC V6T 1Z3 Canada. H These authors contributed equally to this work. Correspondence Steven JM Jones. Email sjones@ Published II September 2009 Received 5 June 2009 Genome Biology 2009 10 R94 doi gb-2009- 10-9-r94 Accepted 11 September 2009 The electronic version of this article is the complete one and can be found online at http 2009 10 9 R94 2009 DiGuistini et al. licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft genome sequences of eukaryotic organisms that integrates sequence information from different sources and demonstrate its effectiveness by assembling an approximately Mb draft genome sequence for the forest pathogen Grosmannia clavigera an ascomycete fungus. We also developed a method for assessing draft assemblies using Illumina paired end read