tailieunhanh - Báo cáo y học: "Tissue-specific spatial organization of genomes"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Tissue-specific spatial organization of genomes. | Research Tissue-specific spatial organization of genomes Luis A Parada Philip G McQueen and Tom Misteli Open Access Addresses National Cancer Institute National Institutes of Health Bethesda MD 20892 USA. Mathematical and Statistical Laboratory Division of Computational Biology Center for Information Technology National Institutes of Health Bethesda MD 20892 USA. Correspondence Tom Misteli. E-mail mistelit@ Published 21 June 2004 Genome Biology 2004 5 R44 The electronic version of this article is the complete one and can be found online at http 2004 5 7 R44 Received 21 April 2004 Revised 24 May 2004 Accepted 25 May 2004 2004 Parada et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background Genomes are organized in vivo in the form of chromosomes. Each chromosome occupies a distinct nuclear subvolume in the form of a chromosome territory. The spatial positioning of chromosomes within the interphase nucleus is often nonrandom. It is unclear whether the nonrandom spatial arrangement of chromosomes is conserved among tissues or whether spatial genome organization is tissue-specific. Results Using two-dimensional and three-dimensional fluorescence in situ hybridization we have carried out a systematic analysis of the spatial positioning of a subset of mouse chromosomes in several tissues. We show that chromosomes exhibit tissue-specific organization. Chromosomes are distributed tissue-specifically with respect to their position relative to the center of the nucleus and also relative to each other. Subsets of chromosomes form distinct types of spatial clusters in different tissues and the relative distance between chromosome pairs varies among tissues. Consistent with the notion that nonrandom spatial proximity is functionally relevant in .