tailieunhanh - Báo cáo y học: "Analysis of alternative splicing with microarrays: successes and challenges"

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Wertheim cung cấp cho các bạn kiến thức về ngành y đề tài: Analysis of alternative splicing with microarrays: successes and challenges. | Minireview Analysis of alternative splicing with microarrays successes and challenges Christopher Lee and Meenakshi Roy Address Molecular Biology Institute Center for Genomics and Proteomics Department of Chemistry and Biochemistry University of California Los Angeles CA 90095-1570 USA. Correspondence Christopher Lee. E-mail leec@ Published 21 June 2004 Genome Biology 2004 5 231 The electronic version of this article is the complete one and can be found online at http 2004 5 7 23l 2004 BioMed Central Ltd Abstract Recently DNA microarrays have emerged as potentially powerful tools for analyzing alternative splicing. We briefly review the latest results in this field and highlight the current challenges that they have revealed. The field of genomics is sometimes accused of being largely a numbers game - increasing our knowledge quantitatively without adding qualitatively to our conceptual understanding. But sometimes big numbers change our mental models. One area in which genomic data appear to be causing just such a shift is the field of alternative splicing. The one gene one product dogma of molecular biology is yielding in the face of large amounts of human genome data to most genes have multiple products with important implications throughout biology 1-6 . Recently several large-scale studies 7-9 have shown that alternative splicing can be analyzed in a high-throughput manner using DNA-microarray methods an approach that is likely to be useful for understanding the role of alternative splicing in many areas of biology. Bioinformatic analyses of expressed sequence tag EST data were the first to herald the alternative-splicing revolution. A number of studies by different groups all reported finding alternative splice forms in a surprisingly large fraction of human genes ranging from 40 to 60 10-15 . These studies have identified more than 30 000 alternative splice forms in human effectively doubling the number of human gene products .