tailieunhanh - Báo cáo khoa học: "Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunohistochemical identification and quantitative analysis of cytoplasmic Cu/Zn superoxide dismutase in mouse organogenesis | J. Vet. Sci. 2008 9 3 233-240 JOURNAL OF Veterinary Science Immunohistochemical identification and quantitative analysis of cytoplasmic Cu Zn superoxide dismutase in mouse organogenesis Jung-Min Yon1 In-Jeoung Baek1 Se-Ra Lee2 Mi-Ra Kim1 Beom Jun Lee1 Young Won Yun1 Sang-Yoon Nam1 College of Veterinary Medicine and Research Institute of Veterinary Medicine Chungbuk National University Cheongju 361-763 Korea 2CKD Research Institute Chong Kun Dang Pharm. Chonan . Box 74 Chonan 330-600 Korea Cytoplasmic Cu Zn superoxide dismutase SOD1 is an antioxidant enzyme that converts superoxide to hydrogen peroxide in cells. Its spatial distribution matches that of superoxide production allowing it to protect cells from oxidative stress. SOD1 deficiencies result in embryonic lethality and a wide range of pathologies in mice but little is known about normal SOD1 protein expression in developing embryos. In this study the expression pattern of SOD1 was investigated in post-implantation mouse embryos and extraembryonic tissues including placenta using Western blotting and immunohistochemical analyses. SOD1 was detected in embryos and extraembryonic tissues from embryonic day ED to . The signal in embryos was observed at the lowest level on ED and the highest level on ED while levels remained constant in the surrounding extraembryonic tissues during all developmental stages examined. Immunohistochemical analysis of SOD1 expression on ED revealed its ubiquitous distribution throughout developing organs. In particular high levels of SOD1 expression were observed in the ependymal epithelium of the choroid plexus ganglia sensory cells of the olfactory and vestibulocochlear epithelia blood cells and vessels hepatocytes and hematopoietic cells of the liver lymph nodes osteogenic tissues and skin. Thus SOD1 is highly expressed at late stages of embryonic development in a cell- and tissue-specific manner and can function as an important antioxidant .

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