tailieunhanh - Báo cáo khoa học: "Immunolocalization of anion exchanger 1 (Band 3) in the renal collecting duct of the common marmoset"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunolocalization of anion exchanger 1 (Band 3) in the renal collecting duct of the common marmoset | J. Vet. Sci. 2007 8 4 329-333 JOURNAL OF Veterinary Science Immunolocalization of anion exchanger 1 Band 3 in the renal collecting duct of the common marmoset 1 1 2 2 3 4 Ji-Hyun Song Yong Hwan Kim Tae-Cheon Kang Moo-Ho Won Jun-Gyo Suh Byung-Hwa Hyun Yang-Seok Oh3 Si-Yun Ryu1 Ju-Young Jung1 2 Department of Veterinary Medicine and Institute of Veterinary Science Chungnam National University Daejeon 305-764 Korea 2Department of Anatomy and 3Department of Human Genetics College of Medicine Hallym University Chuncheon 200-702 Korea 4Genetic Resources Center Korea Research Institute of Bioscience Biotechnology Daejeon 305-806 Korea The purpose of this study was to determine the expression and distribution of band 3 in the collecting duct and connecting tubules of the kidney of the marmoset monkey Callithrix jacchus and to establish whether band 3 is expressed in type A intercalated cells. The intracellular localization of band 3 in the different populations of intercalated cells was determined by double-labeling immunohistochemistry. Immunohistochemical microscopy demonstrated that band 3 is located in the basolateral plasma membranes of all type A intercalated cells in the connecting tubule CNT cortical collecting duct CCD and outer medullary collecting duct OMCD of the marmoset. However type B intercalated cells and non-A non-B intercalated cells did not show band 3 labeling. Electron microscopy of the CNT CCD and OMCD confirmed the light microscopic observation of the basolateral plasma membrane staining for band 3 in a subpopulation of interacted cells. Basolateral staining was seen on the plasma membrane and small coated vesicles in the perinuclear structure some of which were located in the Golgi region. In addition there was no labeling of band 3 in the mitochondria of the CNT CCD and in OMCD cells. The intensity of the immunostaining of the basolateral membrane was less in the CNT than in the CCD and OMCD. In contrast band 3 immunoreactivity was greater in the .