tailieunhanh - Acute Ischemic Stroke Part 5

Tham khảo tài liệu 'acute ischemic stroke part 5', khoa học tự nhiên, công nghệ sinh học phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả | Neuro-EPO by Nasal Route as a Neuroprotective Therapy in Brain Ischemia 61 Fig. 1. Simplified diagram showing the main actors associated with neuronal protective response mediated by HIF-1. Where participating EPO EPOR HIF1a dimerizes with HIF-1b is the signal for transcription in the nucleus and results in EPO mRNA and finally to the synthesis of erythropoietin EPO . Assay in vivo or in vitro Models Rout dose Reference In vitro Primary Culture of Astrocytes Rat 5-20 U mL Diaz Z 2005 Focal and global cerebral ischemia Mouse IP 25-100U Marti HH 2004 Focal Ischemia MCA Rat IN 12 24 U kg Yu YP et al 2005 Focal Ischemia MCA Rat IP 100 1000 5000 U kg Belayev L et al 2005 Retinal Ischemia Mouse and rat IP 5000 U Grimm C et al 2006 Neonatal brain hypoxia Rat IP 1000 U kg Yamada M et al 2011 Spinal cord injury Mouse and rat IP 5000 U Mofidi A et al 2011 Focal cerebral ischemia Gerbils IN U Rodríguez Y et al 2010 Table 1. Some reports of applications of rHu-EPO in cytoprotection. U Unit IP Intraperitoneal Injection MCA Middle Cerebral Artery IN Intra Nasal 3. Brain protection by erythropoietin It has been demonstrated that EPO and its receptor are expressed in brain tissue and their expression increases during ischemia suggesting that they are involved in an endogenous neuroprotective system in mammalian brain 25 . 62 Acute Ischemic Stroke The neuroprotective efficacy of rHu-EPO has been tested in several animal models of nervous system injury in mouse rat gerbil and rabbit including focal and global cerebral ischemia Table 1 showing a reduction of neuronal death. Although the neuroprotective mechanism of rHu-EPO is still being investigated it is known that this effect is mediated by receptors located at the walls of the vascular endothelia and astrocytes 26 . The neuroprotective mechanism of rHu-EPO seems to be multifactor. rHu-EPO may indirectly mediate neuroprotection by restoring the blood supply to the injured tissue or acts directly over the neurons by .

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