tailieunhanh - Báo cáo khoa học: "Aquaporin 1 expression in tissues of canines possessing inherited high K+ erythrocytes"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Aquaporin 1 expression in tissues of canines possessing inherited high K+ erythrocytes | J. Vet. Sci. 2008 9 2 203-205 JOURNAL OF Short Communication Veterinary Science Aquaporin 1 expression in tissues of canines possessing inherited high K erythrocytes Hideharu Ochiai1 Nobuya Hishiyama2 Shin Hisamatsu3 Nobuyuki Kanemaki4 Research Institute of Biosciences 2Laboratory of Pathobiochemistry School of Veterinary Medicine and laboratory of Environmental Chemistry College of Environmental Health and 4Veterinary Teaching Hospital Azabu University Kanagawa 229-8501 Japan We investigated the expression of aquaporin 1 AQP1 in tissues from canines with an inherited anomaly that causes their erythrocytes to have high K . Northern blot analysis revealed abundant AQP1 expression in lung and kidney though little expression was found in spleen. Using antiC-terminus for dog AQP1 abundant expression was shown in kidney trachea and eye but little expression was shown in pancreas and cerebrum indicating that AQP1 expression in canine tissues is similar to that noted in other mammals. Keywords aquaporin 1 expression canine erythrocyte Aquaporins AQP are expressed in a variety of water-transporting epithelia and in many other tissues in which they play an important role in facilitating water transport across the cell membrane. The AQP1 water channel was first isolated from human red blood cells RBCs 2 and was characterized to function as a water channel with high osmotic water permeability 15 . In human erythroleukemia HEL and K562 cells AQP1 expression has been induced by sodium butyrate which is a strong inducer of erythroid differentiation 16 a putative butyrate-re-sponse element has been identified in the promoter sequence of the human AQP1 gene. AQP1 expression has been induced by dimethyl sulfoxide and corticosteroids in mouse erythroleukemia MEL cells 12 . Although a great deal of information is known about AQP1 expression in humans and rodents 1 8 information is quite limited in canines. Previously we determined the cDNA sequence in canine erythroblasts and .