tailieunhanh - Báo cáo khoa học: "Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea | J. Vet. Sci. 2008 9 1 85-90 JOURNAL OF Veterinary Science Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea 1_1 1 1 1 1 1 Seong Joon Joh Chae Ik Shon Sung Won Kang Byoung Han Kim Byung Yul Jeong Kyung Gi Lee Jun Hun Kwon1 Gang Jun Heo2 National Veterinary Research and Quarantine Service Anyang 430-824 Korea 2College ofVeterinary Medicine and Research Institute of Veterinary Medicine Chungbuk National University Cheongju 361-763 Korea The cDNA nucleotide sequence of genome segment B encoding the VP1 protein was determined for the aquatic birnavirus GC1 isolated from the rockfish Sebastes schlegeli in Korea. The VP1 protein of GC1 contains a 2 538 bp open reading frame which encodes a protein comprising 846 amino acid residues that has a predicted MW of 94 kDa. The sequence contains 6 potential Asn-X-Ser Thr motifs. Eight potential Ser phosphorylation sites and 1 potential Tyr phophorylation site were also identified. GC1 contains the Leu-Lys-Asn LKN motif instead of the typical Gly-Asp-Asp GDD motif found in other aquatic birnaviruses. We also identified the GLPYIGKT motif the putative GTP-binding site at amino acid position 248. In total the VP1 regions of 22 birnavirus strains were compared for analyzing the genetic relationship among the family Birnaviridae. Based on the deduced amino acid sequences GC1 was observed to be more closely related to the infectious pancreatic necrosis virus IPNV from the USA Japan and Korea than the IPNV from Europe. Further aquatic birnaviruses containing GC1 and IPNV have genogroups that are distinct from those in the genus Avibirnaviruses and Entomo-birnaviruses. The birnavirusstrains were clustered into 5 genogroups based on their amino acid sequences. The marine aquatic birnaviruses MABVs containing GC1 were included in the MABV genogroup the IPNV strains isolated from Korea Japan and the USA were included in genogroup 1 and the IPNV strains isolated .