tailieunhanh - Báo cáo khoa học: " Evaluation of serum chondroitin sulfate and hyaluronan: biomarkers for osteoarthritis in canine hip dysplasia"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Evaluation of serum chondroitin sulfate and hyaluronan: biomarkers for osteoarthritis in canine hip dysplasia | J. Vet. Sci. 2008 9 3 317-325 JOURNAL OF Veterinary Science Evaluation of serum chondroitin sulfate and hyaluronan biomarkers for osteoarthritis in canine hip dysplasia Korakot Nganvongpanit1 2 Akanit Itthiarbha2 Siriwan Ong-Chai2 Prachya Kongtawelert2 Department of Veterinary Preclinical Science Faculty of Veterinary Medicine Chiang Mai Universily Chiang Mai 50100 Thailand 2Thailand Excellence Center for Tissue Engineering Department of Biochemistry Faculty of Medicine Chiang Mai University Chiang Mai 50200 Thailand Hip dysplasia HD is one of the most important bone and joint diseases in dogs. Making the radiographic diagnosis is sometime possible when the disease has markedly progressed. Chondroitin sulfate CS and hyaluronan HA are the most important cartilage biomolecules that are elevated in the serum taken from dogs with osteoarthritis. The serum CS and HA can be detected by an ELISA technique with using monoclonal antibodies against CS epitope 3B3 and WF6 and the HA chain as the primary antibodies. The aim of this study was to compare the levels of serum CS both epitopes and HA in non-HD and HD dogs. All 123 dogs were categorized into 2 groups. The non-HD group was composed of 98 healthy dogs while the HD group was comprised of 25 HD dogs. Blood samples were collected for analyzing the serum CS and HA levels with using the ELISA technique. The results showed that the average serum level of the CS epitope WF6 in the HD group 2 594 3 ng ml was significantly higher than that in the non-HD group 465 ng ml p while the epitope 3B3 in the HD group 105 ng ml was significantly lower than that in the non-HD group 136 ng ml p . The amount of serum HA in the HD group ng ml was lower than that in the non HD group ng ml p . The results indicate that the serum CS and HA levels might be used as biomarkers for osteoarthritis in HD dogs. Keywords biomarker chondroitin sulfate dog hip dysplasia osteoarthritis