tailieunhanh - Báo cáo khoa học: " Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin- neuraminidase proteins"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin- neuraminidase proteins | J. Vet. Sci. 2008 9 3 301-308 JOURNAL OF Veterinary Science Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutininneuraminidase proteins 1 2 1 1 1 3 Youn-Jeong Lee Haan-Woo Sung Jun-Gu Choi Eun-Kyoung Lee Hachung Yoon Jae-Hong Kim Chang-Seon Song4 A National Veterinary Research and Quarantine Service Anyang 430-824 Korea 2School of Veterinary Medicine Kangwon National University Chunchon 200-701 Korea 3College of Veterinary Medicine Seoul National University Seoul 151-742 Korea 4College of Veterinary Medicine Konkuk Univdersity Seoul 143-701 Korea Recombinant baculoviruses containing the fusion F and hemagglutinin-neuraminidase HN glycoprotein gene of the viscerotropic velogenic vv Newcastle disease virus NDV isolate Kr-005 00 and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit vaccine to the recent epizootic vvNDV. The level of protection was determined by evaluating the clinical signs mortality and virus shedding from the oropharynx and cloaca of chickens after a challenge with vvNDV Kr-005 00. The recombinant ND F rND F and recombinant HN rND HN glycoproteins derived from the velogenic strain provided good protection against the clinical signs and mortality showing a PI value and 100 protection after a booster immunization. On the other hand the combined rND F HN glycoprotein derived from the velogenic strain induced complete protection PI value and 100 protection and significantly reduced the amount of virus shedding even after a single immunization. The rND F and rND HN glycoproteins derived from the velogenic strain had a slightly but not significantly greater protective effect than the lentogenic strain. These results suggest that the combined rND F HN glycoprotein derived from vvNDV can be an ideal subunit marker vaccine candidate in chickens in a future ND eradication program. Keywords Newcastle disease recombinant .