tailieunhanh - Báo cáo khoa học: "Serum immunoglobulin fused interferon-Ձ inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Serum immunoglobulin fused interferon-Ձ inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells | J. Vet. Sci. 2008 9 1 45-50 JOURNAL OF Veterinary Science Serum immunoglobulin fused interferon-a inhibited tumor growth in athymic mice bearing colon 26 adenocarcinoma cells Jun-Sung Kim1 Kyeong Nam Yu1 Mi Suk Noh1 2 Min-Ah Woo1 2 Sung-Jin Park1 Jin Hong Park1 Jin Hua1 IT__ri__ I_1 o_T7__IT_1 ÍT _ ri_T_1x7 o_ I_1 X_X7yi 1_ 3 ri_ _TZ _3 Hyun Sun Cho Soon Kyung Hwang Eun-Sun Lee Youn-Sun Chung In-Young Choi Se-Chang Kwon Myung-Haing Cho1 2 laboratory of Toxicology College of Veterinary Medicine and 2Interdisciplinary Program in Nano-Science and Technology Seoul National University Seoul 151 -742 Korea 3Hanmi Pharmaceutical Research Center Hwaseong 445-813 Korea Interferon IFN has therapeutic potential for a wide range of infectious and proliferative disorders. However the half-life of IFN is too short to have a stable therapeutic effect. To overcome this problem serum immunoglobulin has been fused to IFN. In this study the efficacy of serum immunoglobulin fused INFs si-IFN1 and si-IFN2 was evaluated on athymic mice bearing colon 26 adenocarcinoma cells. Seven days after the implantation of tumor cells each group of mice was injected once a week with si-IFN1 and si-IFN2 at two different concentrations 10 X 30 pg kg and 50 X 150 pg kg . A slight anti-tumoral effect was observed in all 10 X groups compared to the control. In the 50 X groups however si-IFN1 and si-IFN2 showed significant anti- tumoral effects compared to the control. To gain more information on the mechanisms associated with the decrease of tumor size a Western blot assay of apoptosis-related molecules was performed. The protein expression of cytochrome c caspase 9 6 and 3 were increased by si-IFN1 and si-IFN2. These 2 IFNs also increased the expressions of p53 p21 Bax and Bad. Interestingly si-IFN1 and si-IFN2 decreased the expression of VEGF-p. Taken together serum immunoglobulin fused IFNs increased therapeutic efficacy under current experimental condition. Keywoids adenocarcinoma cell .