tailieunhanh - Báo cáo khoa học: "Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease | J. Vet. Sci. 2008 9 1 15-23 JOURNAL OF Veterinary Science Lesions in the thymus and bone marrow in chicks with experimentally induced chicken infectious anemia disease Burak Kuscu Aydin Gtìrel Department of Pathology Faculty of Veterinary Medicine Istanbul University Avcilar 34310 Istanbul Turkey One-day-old SPF chicks were inoculated with the Cux-l strain of chicken infectious anemia virus CIAV and the clinical development of disease and its macroscopic and microscopic alterations in the thymus and bone marrow were observed. Tissue sections of thymus and bone marrow were stained using the streptavidin-biotin peroxidase method and examined under light microscope for evaluation of antigenic intensities in tissues. Those findings were then compared with blood parameters and ELISA results obtained through collected sera during sacrifice procedures. We sought to determine the localization of viral antigens in thymus and bone marrow tissues after inoculation the correlation between antigen intensities and hematologic serologic and histopathologic findings definitive diagnostic criteria using histopathologic and immunoperoxidase methods and the reliability of these methods in the diagnosis of CIAV infection. For this purpose 83 one-day-old SPF chicks were used. The birds were divided into experimental n 52 and control n 26 groups. A virus dose of TCID50 of 100 000 ml was administered intramuscularly to every bird in the experimental group. Based on the results of this study we have suggested that clinical examination along with macroscopic and microscopic evaluation of the thymus and bone marrow maybe undertaken starting from day 7 post-inoculation PI . ELISA might be of value as it might give consistent results starting from day 14 PI. However the most reliable results were obtained through examination of thymus and bone marrow sections from infected birds stained by immunoperoxidase technique as early as day 4 PI. Keywords bone marrow chicken infectious anemia .