tailieunhanh - Báo cáo khoa học: "Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal | J. Vet. Sci. 2007 8 4 415-421 JOURNAL OF Veterinary Science Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal Eunsong Lee1 Kilyoung Song2 School of Veterinary Medicine and Institute of Veterinary Science Kangwon National University Chunchon 200-701 Korea 2College of Veterinary Medicine Seoul National University Seoul 151-742 Korea The objective of the present study was to examine the feasibility of the production of autologous porcine somatic cell nuclear transfer SCNT blastocysts using oocytes and donor cells from slaughtered ovaries. Therefore we attempted to optimize autologous SCNT by examining the effects of electrical fusion conditions and donor cell type on cell fusion and the development of SCNT embryos. Four types of donor cells were used 1 denuded cumulus cells DCCs collected from in viírơ-matured IVM oocytes 2 cumulus cells collected from oocytes after 22 h of IVM and cultured for 18 h CCCs 3 follicular cells obtained from follicular contents and cultured for 40 h CFCs and 4 adult skin fibroblasts. The DCCs showed a significantly p lower rate of fusion than the CCCs when two pulses of 170 V mm DC were applied for 50 gsec 19 2 vs. 77 3 . The rate of DCC fusion with oocytes was increased by the application of two DC pulses of 190 V mm for 30 gsec although this was still lower than the rate of fusion in the CCCs 33 1 vs. 80 2 . The rates of cleavage 57 5 and blastocyst formation 1 1 in the DCC-derived embryos did not differ from those 55 6 and 3 1 respectively in the CCC-de-rived SCNT embryos. Autologous SCNT embryos derived from CFCs 5 2 showed higher levels of blastocyst formation p than CCC-derived autologous SCNT embryos 1 0 . In conclusion the results of the present study show that culturing cumulus and follicular cells before SCNT enhances cell fusion with oocytes and that CFCs are superior to CCCs in the production of higher numbers of autologous SCNT blastocysts. Key words .