tailieunhanh - Báo cáo khoa học: " Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea | J. Vet. Sci. 2007 8 4 401-407 JOURNAL OF Veterinary Science Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses identification of a novel phylogenetic group in Korea Ji-Hyun Jang1 Haan-Woo Sung1 Chang-Seon Song2 Hyuk-Moo Kwon1 School ofVeterinary Medicine and Institute ofVeterinary Science Kangwon National Universily Chuncheon 200-701 Korea 2College of Veterinary Medicine Konkuk University Seoul 143-701 Korea Twelve Korean infectious bronchitis viruses IBVs were isolated in the field from chickens suspected of being carriers of infectious bronchitis between 2001 and 2003. The S1 glycoprotein genes of these IBV isolates were amplified by reverse transcriptase-polymerase chain reaction RT-PCR and analyzed by restriction fragment length polymorphism RFLP analysis. These Korean IBV isolates were classified into three groups according to their RFLP patterns obtained using the restriction enzyme HaeIII. Half of the twelve isolates were similar to the KM91 RFLP pattern which is a common pattern in Korea. Three more isolates were related to the Arkansas strain pattern but with some unique variations. The other three viruses showed variant RFLP patterns. For a comparison with the published sequences for non-Korean IBV strains amplified PCR products from the twelve isolates were cloned and sequenced. The Korean IBV field isolates had nucleotide sequence homology with each other and nucleotide sequence homology with non-Korean IBV strains. With respect to the deduced amino acid sequence the Korean IBV isolates had similarity with each other and similarity with nonKorean IBV strains. Phylogenetic tree analysis revealed that some of the IBV isolates appear to belong to a new group different from the non-Korean IBV strains or from previously isolated Korean IBV strains. Specifically the new Korean IBV isolates K10217-03 K3-3 and K1255-03 represented a separate group. These findings suggest that the Korean IBVs .