tailieunhanh - Báo cáo khoa hoc:" François Hatey sequence a tags for"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: François Hatey sequence a tags for | 521 Genet. Sei. Evol. 30 1998 521-541 Inra Elsevier Paris Review Expressed sequence tags for genes a review Francois Hatey Gwenola Tosser-Klopp Catherine Clouscard-Martinato Philippe Mulsant Fran go is Gasser Laboratoire de génétique cellulaire Institut national de la recherche agronomique BP 27 31326 Castanet-Tolosan cedex France Received 16 September 1997 accepted 7 July 1998 Abstract - Expressed sequence tags ESTs are partial sequences from the extremities of complementary DNA cDNA resulting from a single pass sequencing of clones from cDNA libraries and different ESTs can be obtained from one gene. Sequence information from ESTs can be used for deciphering the function and the organisation of the genome. From a functional viewpoint they allow the determination of the expression profiles of genes in any particular tissue in different conditions or status and thus the identification of regulated genes. In order to identify genes involved in particular processes one can select a specific group of mRNAs. For such a selection classical techniques include subtraction or differential screening and new techniques using polymerase chain reaction PCR amplification are now available. For studies on the organisation of the genome the main use of ESTs is the determination of chromosomal localisation of the corresponding genes using a somatic hybrid cell panel. This chromosomal localisation information is needed to identify genes or quantitative trait loci according to the positional candidate approach. ESTs also contribute to comparative genetics and they can help to decipher gene function by comparison between species even genetically distant ones. Thus combining sequence functional and localisation data ESTs contribute to an integrated approach to the genome. Inra Elsevier Paris expressed sequence tags functional genomics gene mapping comparative genetics Resume - Des etiquettes pour les genes une revue. Les etiquettes correspondent aux sequences des extrémités des ADN .