tailieunhanh - Báo cáo khoa hoc:" Comparative influence of Odh and Adh loci on alcohol tolerance in Drosophila melanogaster"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: Comparative influence of Odh and Adh loci on alcohol tolerance in Drosophila melanogaster | Genet. Sei. Evol. 30 1998 503-516 Inra Elsevier Paris 503 Original article Comparative influence of Odh and Adh loci on alcohol tolerance in Drosophila melanogaster Katalin Bokor Katalin Pecsenye Department of Evolutionary Zoology Kossuth L. University Debrecen H-4010 Hungary Received 28 November 1997 accepted 9 September 1998 Abstract - The effect of ethanol on larva-to-pupa and larva-to-adult survival was compared in ten laboratory strains of Drosophila melanogaster. The strains had five different allele combinations at the Adh and Odh loci. Two parallel strains of the five two-locus genotypes were isolated from different isofemale lines and so they had different genetic backgrounds. Second instar larvae of all ten strains were exposed to different ethanol treatments and larva-to-pupa and larva-to-adult survival components were estimated. The strains with different genetic background but identical genotypic combinations at the Adh and Odh loci had different initial survival rates but they exhibited similar tolerance to ethanol. Ethanol tolerance appeared to depend predominantly on the Odh locus. The two Adh genotypes did not show significantly different ethanol tolerance. In contrast the three Odh genotypes tolerated exogenous ethanol differently OdhF homozygotes had the highest tolerance to ethanol in both the larval and pupal stages. Inra Elsevier Paris Drosophila melanogaster Adh I Odh alcohol tolerance Resume Le locus Odh et la souche génétique ont plus d influence sur la tolerance à 1 alcool que le locus Adh chez Drosophila melanogaster. L influence de 1 éthanol sur la survie du stade larvaire à la nymphose et de la nymphose à 1 état imago a été comparée dans dix souches de Drosophila melanogaster. Les souches présentaient cinq combinaisons alléliques aux locus Adh et Odh. Pour chacun des cinq genotypes deux souches ont été isolées à partir de lignée isofemelles differentes c est-à-dire qu elles possédaient des fonds génétiques differents. Les larves de .