tailieunhanh - Báo cáo sinh học: "Chromosomal analysis of embryos produced by artificially inseminated superovulated cattle"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Chromosomal analysis of embryos produced by artificially inseminated superovulated cattle | 189 Genet Sei Evol 1995 27 189-194 Elsevier INRA Note Chromosomal analysis of embryos produced by artificially inseminated superovulated cattle WA King1 2 A Verini Supplizi2 3 HEP Diop2 4 D Bousquet5 1 University of Guelph Department of Biomedical Sciences Ontario Veterinary College Guelph ON NIG 2W1 Canada . 2 Université de Montreal centre de recherche en reproduction animate faculte de médecine vétérinaire CP5000 Saint-Hyacinthe PQ J2S 7C6 Canada 3 Instituto di Produzioni Animali Facolta de Medicina Veterinaria Via San Costanzo 4 06100 Perugia Italy 4 École inter-États des sciences et médecine vétérinaire departement Chirurgie et Reproduction BP 5077 Dakar Senegal 5 BOVITEQ 1425 Grand Rang Saint-Frangois Saint-Hyacinthe PQ J2S 7A9 Canada Received 23 June 1994 accepted 14 November 1995 Summary - To determine the chromosomal complement of pre-morula bovine embryos 30 Holstein heifers were superovulated and inseminated and their embryos were subjected to chromosome analysis on days 2 3 or 4. Of the 298 embryos ova recovered 101 had one or more cells in metaphase and of these 60 could be karyotyped. Eight of the karyotyped embryos were chromosomally abnormal including 3 triploids 1 tetrapioid and 4 mixopioids. It was concluded that the abnormalities occurred at or shortly after the time of fertilization. bovine embryo chromosome triploid tetrapioid mixopioid Resume - Analyse chromosomique chez des embryons provenant de vaches super-ovulées et inséminées artificiellement. Afin de determiner la garniture chromosomique d embryons bavins aux stades de pré-morula 30 génisses Holstein ont été superovulées et inséminées. Les embryons furent récoltés aux jours 2 3 OU 4 et soumis à une analyse chromosomique. Sur un total de 298 embryons ovules récoltés 101 montraient une OU plusieurs cellules en metaphase et 60 de ceux-ci ont pu être examines pour leur caryotype. Huit embryons analyses présentaient une garniture chromosomique anormale 3 triploides 1 tétraploĩde et 4 mixoploi