tailieunhanh - Báo cáo khoa học: "Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting, antibiotic resistance and plasmid profiles | J. Vet. Set. 2006 7 1 37-41 JOURNAL OF Veterinary Science Analysis of Salmonella enterica serotype Enteritidis isolated from human and chickens by repetitive sequence-PCR fingerprinting antibiotic resistance and plasmid profiles Dong Kyun Suh1 Jae Chan Song2 1 Research Institute of Health and Environment Daegu 706-841 Korea 2College of Veterinary Medicine Kyungpook National University Daegu 702-701 Korea A total of 22 Salmonella enterica serotype Enteritidis A. Enteritidis strains isolated from human and chicken were subjected to DNA fingerprinting by repetitive sequence PCR using ERIC and BOX primers antibiotic resistance and plasmid patterns. Both ERIC and BOX PCR amplification data revealed a highly genetic homogeneity between isolates from human and chicken except one isolate which originated from chicken and showed a different DNA band pattern from others. Eleven of 22 X. Enteritidis isolates 50 were resistant to more than one antibiotics and characterized by 5 resistance patterns. The most common pattern was penicillin resistant . Only one isolate from chicken showed a multiple drug resistance patterns to 4 antibiotics. All 22 X. Enteritidis isolates harbored more than two plasmids with eight different plasmid profiles including two to six plasmids with approximate molecular size ranging from to 21 kb. A band of 15 kb size was detected in all isolates tested however the band sizes smaller than 15 kb were found only in isolates from chicken. Key words antibiotic resistance plasmid rep-PCR fingerprinting Salmonella enterica serotype Enteritidis Introduction Salmonella enterica serotype Enteritidis S. Enteritidis is one of the most important serotypes causing human gastroenteritis outbreaks worldwide during the last few decades. Animals and thefr products particularly meat and eggs from chicken were considered major sources of infections with this pathogen for human 17 . Because of the importance of Salmonella in food-borne diseases many Corresponding .