tailieunhanh - Báo cáo khoa học: "Increased phosphorylation of c-Jun NH (2)-terminal protein kinase in the sciatic nerves of Lewis rats with experimental autoimmune neuritis"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Increased phosphorylation of c-Jun NH (2)-terminal protein kinase in the sciatic nerves of Lewis rats with experimental autoimmune neuritis | J. Vet. Set. 2006 7 1 13-17 JOURNAL OF Veterinary Science Increased phosphorylation of C-Jun NH 2 -terminal protein kinase in the sciatic nerves of Lewis rats with experimental autoimmune neuritis Meejung Ahn Taekyun Shin Department of Veterinary Medicine Cheju National University Jeju 690-756 Korea The phosphorylation of C-Jun Nil 2 -terminal protein kinase JNK one of the mitogen-activated protein kinases was analyzed in the sciatic nerves of Lewis rats with experimental autoimmune neuritis EAN . Western blot analysis showed that the expression levels of both phosphorylated .INK I p-JNK I approximately 46 kDa and phosphorylated JNK2 p-JNK2 approximately 54 kDa in the sciatic nerves of rats with EAN increased significantly p at day 14 post-immunization PI and remained at this level at days 24 and 30 PI with a slight decrease. In EAN-affected sciatic nerves there was intense immunostaining for p-JNK in the infiltrating inflammatory cells especially ED1-positive macrophages and Schwann cells on days 14-24 PI compared with those of controls. Some macrophages with increased p-JNK immunoreactivity was shown to be apoptotic while some Schwann cells remained survived in this rat EAN model suggesting that .INK is differentially involved in the EAN-affected sciatic nerves. These findings suggest that JNK phosphorylation is closely associated with the clearance of inflammatory cells as well as the activation of Schwann cells in the EAN affected sciatic nerves. Key words apoptosis autoimmune neuritis demyelination INK sciatic nerve The C-Jun NH 2 -tenninal protein kinase INK also called the stress-activated protein kinase SAPK is one of three subgroups of the mitogen-activated protein MAP kinase family 2 JNK activation plays essential roles in organogenesis during mouse development by regulating cell proliferation survival or apoptosis and in immune responses by regulating cytokine gene expression 10 . The phosphorylation of JNK p-JNK may stimulate transcription factors