tailieunhanh - Báo cáo khoa học: "Characterization of Brachyspira hyodysenteriae isolates from Korea"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Characterization of Brachyspira hyodysenteriae isolates from Korea | J. Vet. Set. 2005 6 4 335-339 JOURNAL OF Veterinary Science Characterization of Brachyspira hyodysenteriae isolates from Korea Tae Jung Kim1 Suk Chan Jung2 Jae II Lee1 1 College of Veterinary Medicine Chonnam National University Gwangju 500-757 Korea 2 National Veterinary Research and Quarantine Service Ministry of Agriculture and Forestry Anyang 430-826 Korea This study was done to characterize diversity in 10 Brachyspira hyodysenteriae isolates in Korea. The isolates were compared with 14 well-characterized non-Korean strains of various Brachyspira species. All Korean isolates showed strong beta haemolysis and had blunt cell ends with 7 14 periplasmic flagella. They produced indole and did not ferment fructose. They were alpha-glucosidase positive and alpha-galatosidase negative using the API-ZYM kit. Using polyclonal antisera raised in rabbits against recognized serotypes all isolates showed a strong reaction to B. hyodysenteriae antisera E A and B. Using multilocus enzyme electrophoresis MLEE with 15 enzymes and 5 buffer systems the Korean and non-Korean isolates were divided into 22 electrophoretic types ETs and 5 divisions A B c D and E . Division A corresponded to B. hyodysenteriae B to B. innocens c to B. intermedia D to B. murdochii and E to B. pilosicoli. The 10 Korean isolates of B. hyodysenteriae were relatively diverse being divided into 9 ETs within MLEE division A. They were all distinct from the non-Korean strains. Key words Brachyspira hyodysenteriae multilocus enzyme electrophoresis Korea serotype swine dysentery Introduction Swine dysentery SD is a mucohaemorrhagic colitis of pigs caused by infection with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae 7 . Useful features that can help distinguish B. hyodysenteriae from other related intestinal spirochaete species include thefr ability to produce indole and to ferment fructose thefr enzymatic profile in the commercial API-ZYM kit and the presence of strong beta-haemolysis 3 11 . .