tailieunhanh - Báo cáo sinh học: "A comparison between the β-globin gene clusters of domestic sheep (Ovis aries) and Sardinian mouflon"

A comparison between the β-globin gene clusters of domestic sheep (Ovis aries) and Sardinian mouflon | - _ _ Í NÂỈ A C .. Genet Sei Evol 1996 28 217-222 tfs G4ft4 u Antfflale 217 Elsevier INRA SlgUOTHifiUS _ s. 75352 JOUV BN JftSAS Original article A comparison between the -globin gene clusters of domestic sheep Ovis aries and Sardinian mouflon Ovis gmelini musimon A Rando1 p Di Gregorio2 M Capuano1 c Senese1 L Manca2 s Naitana3 B Masala2 1 Dipartimento di Scienze delle Produzioni Animali ưniversità degli Studi della Basilicata Via N Sauro 85 85100 Potenza 2 Istituto di Fisiologia Generale e Chimica Biologica Università degli Studi di Sassari Via Muroni 25 3 Istituto di Fisiologia degli Animali Domestici Università degli Studi di Sassari Via Vienna 2 07100 Sassari Italy Received 15 March 1995 accepted 15 February 1996 Summary - The organization of the 3-globin gene cluster of wild Sardinian mouflons with different haemoglobin phenotypes has been analyzed by means of Southern blot with probes specific for eIV- and 3F- globin genes. With three endonucleases Bam HI Eco RI and Hind III Sardinian mouflons with Hb B Hb BM and Hb M show identical restriction patterns to sheep with Hb A Hb AB and Hb B respectively. Therefore in Sardinian mouflons as in domestic sheep the 3-globin gene cluster shows two haplotypes characterized by the triplication or duplication of an ancestral four gene set e-e-Tpfl-fl . domestic sheep Sardinian mouflon haemoglobin I fl-globin cluster Resume - Comparaison du groupe de genes de la globine- du mouton domestique Ovis aries et du mouflon de Sardaigne Ovis gmelini musimon . L organisation du groupe de gènes de la globine-Ẹ chez des mouflons de Sardaigne montrant divers phenotypes d hemoglobine a ệté analysee par buvardage de Southern avec des sondes specifiques pour les genes IV et flF. Avec trois endonucleases Bam HI Eco RI et Hind III les mouflons caractérisés par les phenotypes d hemoglobine B BM et M montrent des patrons de restriction identiques aux moutons ayant les phenotypes d hemoglobine A AB et B respectivement. Par consequent le .