tailieunhanh - Báo cáo sinh học: "Induction of chromosomal sites in goats: a fragile preliminary study"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Induction of chromosomal sites in goats: a fragile preliminary study | IJ JUIN 199b Genet Sei Evol 1996 28 129-139 Elsevier INRA 129 Original article Induction of chromosomal fragile sites in goats a preliminary study NL Lopez-Corrales MV Arruga Departamento de Production Animal y Ciencias de los Alimentos Facultad de Veterinaria de la Universidad de Zaragoza c Miguel Servet 177 50013 Zaragoza Spain Received 9 November 1995 accepted 16 January 1996 Summary - The current study describes the results obtained from different methods of detection of folate-sensitive fragile sites in goat chromosomes. Two different types of expression of chromosomal fragility have been observed telomeric non-staining gaps in 20 out of 21 animals studied and chromatidic breaks in ten animals. The non-staining gaps have been identified mainly in chromosome 5 and their frequency of occurrence ranged from 30 to 66 of the cells. The chromatidic break occurrence ranged from 2 to 5 of the cells among the break carriers. From the methods used and the observed frequency of expression in cultures the gaps were classified as common folate-sensitive fragile sites. Significant differences between the induction methods used were obtained. goat fragile site folate deficiency Resume Induction de sites chromosomiques fragiles chez les chèvres étude préliminaire. Cette étude décrit les résultats de differentes methodes de mise en evidence de sites chromosomiques fragiles sensibles au folate chez la chèvre. Deux types differents d expression de la fragilite chromosomique ont été observes des espaces télomériques ne prenant pas la coloration sur 20 des 21 animaux étudiés et des cassures chromatidiques sur 10 animaux. Les absences de coloration ont été localisées principalement sur le chromosome 5 et leur frequence d apparition allait de 30 à 66 des cellules. Chez les por-teurs de cassures la frequence de ces demières allait de 2 à 5 des cellules. D après les methodes utilisées et les frequences observées dans les cultures les zones chromosomiques non colorées peuvent être .