tailieunhanh - Báo cáo khoa học: " An immunohistochemical study of chromogranin A and Sp-1 immunoreactive cells in the gastrointestinal tract of ovariectomized rats"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: An immunohistochemical study of chromogranin A and Sp-1 immunoreactive cells in the gastrointestinal tract of ovariectomized rats | J. Vet. Set. 2005 6 3 191-196 JOURNAL OF Veterinary Science An immunohistochemical study of chromogranin A and Sp-1 immunoreactive cells in the gastrointestinal tract of ovariectomized rats Sae-kwang Ku1 Hyeung-sik Lee2 Jae-hyun Lee3 Pharmacology Toxicology Lab. Central Research Laboratories Dong-Wha Pharm. Ind. Co. Anyang 430-017 Korea department of Herbal Biotechnology Daegu Haany University Daegu 712-715 Korea department of Histology College of Veterinary Medicine Kyungpook National University Daegu 702-701 Korea The changes on the regional distributions and frequencies of two types of chromogranin chromogranin A CGA and bovine Sp-1 chromogranin BCG -immunoreactive IR cells in gastrointestinal GI tract of osteoporotic Sprague-Dawley rat induced by ovariectomy were studied by immunohistochemical methods. The experimental animals were divided into two groups one is non-ovariectomized group Sham and the other is ovariectomized group OVX . Samples were collected from each part of GI tract at 10th week after ovariectomy or sham operation. CGA-IR cells were restricted to the stomach regions with various frequencies regardless of ovariectomy except for the fundus of ovx in which no cells were detected. In addition BCG-IR cells were also restricted to the pylorus and duodenum regardless of ovariectomy. A significantly decrease of CGAIR cells was detected in ovx compared to that of Sham in both fundus and pylorus and BCG-IR cells were also significantly decreased in the duodenum p . However in the pylorus BCG-IR cells in ovx showed similar frequency compared to that of Sham. In conclusion the abnormality in density of chromogranin a generally used GI endocrine cell marker detected in this study may contribute to the development of GI symptoms in osteoporosis such as impairments of calcium and some lipids frequently encountered in patients with postmenopausal osteoporosis. Key words ovariectomy osteoporosis chromogranin endocrine immunohistochemistry Introduction .