tailieunhanh - Báo cáo khoa học: " Expression of placenta growth factor mRNA in the rat placenta during mid-late pregnancy"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Expression of placenta growth factor mRNA in the rat placenta during mid-late pregnancy | J. Vet. Set. 2005 6 3 179-183 JOURNAL OF Veterinary Science Expression of placenta growth factor mRNA in the rat placenta during mid-late pregnancy Wan-Sung Choi1 Gyeong-Jae Cho1 Chung-Kil Won2 Phil-Ok Koh2 Department of Anatomy and Neurobiology College of Medicine and Institute of Health Sciences Gyeongsang National University Jinju 660-751 Korea 2Department of Anatomy College of Veterinary Medicine and Institute of Animal Medicine Gyeongsang National University Jinju 660-701 Korea The placenta is an essential organ that synthesizes several growth and angiogenic factors for its own growth as well as fetal development. It is known that the placenta growth factor P1GF is a member of the vascular endothelial growth factor family and is critical for placental growth and fetal development. However there is little information regarding the expression pattern and cellular localization of P1GF inRNA in rat placenta during pregnancy. The aim of this study was to define the distribution of P1GF inRNA in rat placenta at various gestations. RT-PCR analysis showed that the expression level of P1GF inRNA increased as gestation advanced. Using in situ hybridization histochemistry positive cells of P1GF inRNA were detected in chorionic villi. P1GF inRN A was expressed in the trophoblast cells and stroma cells surrounding the blood vessels within chorionic villi on day 13 and 15. Also positive signals of P1GF inRNA were strongly detected in stroma cells of chorionic villi on day 17 19 and 21. In particular the density and number of positive signals of P1GF inRNA was significantly increased as gestation advanced. The expression pattern of P1GF inRNA in rat placenta during pregnancy demonstrates that P1GF plays a functional role for placental growth and fetal development during mid-late pregnancy. Key words placenta placenta growth factor rat Introduction The placenta is a vital organ for both fetal development and the maintenance of pregnancy. It is the biosynthetic site .