tailieunhanh - Báo cáo khoa học: "Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes | J. Vet. Sci. 2005 6 2 157-160 JOURNAL OF Short Communication Veterinary Science Exploration of immunoblot profiles of Neospora caninum probed with different bovine immunoglobulin classes Yong-Seung Shin Eung-Goo Lee Tae-Sung Jung Institue of Animal Medicine College of Veterinary Medicine GyeongsangNational University Jinju 660-701 Korea 2Department of Molecular Parasitology School of Medicine Sungkyunkwan University Suwon 440-746 Korea The present study was attempted to compare the Neospora caninum N. caninum antigenic bands recognized by different bovine immunoglobulin classes. A total 10 5 2 and 6 antigenic bands were exhibited on immunoblot profiles against bovine IgM IgE IgA and IgG respectively. A 46 kDa band was probed as a common antigenic band except IgA 69 kDa band was bovine IgM and IgE 33 37 55 and 79 kDa bands were bovine IgM and IgG 72 kDa band was found IgM and IgA profiles. Based on the analysis it appeared that different immunoglobulin classes recognizing different antigenic molecules were cooperating to cope with neosporosis. Key words antigenic protein bovine immunoglobulin classes immunoblotting Neospora caninum Neospora caninum N. caninum an obligate intracellular protozoan parasite is known to have a great similarity to Toxoplasma gondii T gondii which is belonging to the same phylum Apicomplexa 4 . This parasite has been identified as a contagious pathogen in animals worldwide particularly cattle and dogs 5 . Antigenic proteins from N. caninum have been identified and tested for diagnosis by a number of investigators 6 7 but there are very few reports available on the humoral immune responses against N. caninum as opposed to T. gondii studied well at different stages of infection. Briefly T. gondii specific IgG usually appears within 1-2 weeks after infection and reaches its peak concentration within 1-2 months after which it gradually declines though it generally persists for life 18 . IgM appears earlier and declines more rapidly than IgG .