tailieunhanh - Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene | J. Vet. Sci. 2005 6 2 151-155 JOURNAL OF Veterinary Science Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR sequencing and phylogenetic analysis based on 16S rRNA gene Seung-Ok Lee1 Dong-Kyeun Na Chul-Min Kim Ying-Hua Li Yoon-Hee Cho Jin-Ho Park John-Hwa Lee1 Seong-Kug Ek Terry A. Klein Joon-Seok Chae1 Bio-Safety Research Institute and College of Veterinary Medicine ChonbukNational University Jeonju 561-756 Korea 2Force Health Protection DCSFHP 18th Medical Command Unit 15821 BOX 754 APO AP 96205-5281 USA Genomic DNAs extracted from 1 288 Haemaphysalis longicornis ticks collected from grass vegetation and various animals from nine provinces of Korea were subjected to screening by genus-specific Ehrlichia spp. or Anaplasma spp. real-time TaqMan PCR and speciesspecific E. chaffeensis nested-PCR based on amplification of 16S rRNA gene fragments. In all 611 ticks tested positive for genus-specific amplification of 116 bp fragment of 16S rRNA of Ehrlichia spp. or Anaplasma spp. Subsequently 396 bp E. chaffeensis-specific fragment of 16S rRNA was amplified from 26 611 tick samples. The comparison of the nucleotide sequence of 16S rRNA gene from one tick EC-PGHL GeneBank accession number AY35042 with the sequences of 20 E. chaffeensis strains available in the database showed that EC-PGHL was 100 identical or similar to the Arkansas AF416764 the Sapulpa U60476 and the 91HE17 U23503 strains. The phylogenetic analysis also revealed that the E. chaffeensis EC-PGHL formed a single cluster with the above strains. This is the first study to report molecular detection and phylogenetic analysis of E. chaffeensis from H. longicornis ticks in Korea. The implicit significance of E. chaffeensis infection in H. longicornis ticks in Korea is discussed. Key words Ehrlichia chaffeensis Haemaphysalis longicornis prevalence PCR Introduction Ehrlichia species are strict intracellular gram-negative bacteria that .