tailieunhanh - Báo cáo khoa học: " Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice | J. Vet. Sci. 2005 6 2 125-133 JOURNAL OF Veterinary Science Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice Dong-Kun Yang Chang-Hee Kweon Byoung-Han Kim Seong-In Lim Jun-Hun Kwon Seong-Hee Kim Jae-Young Song Hong-Ryul Han2 National Veterinary Research and Quarantine Service Ministry of Agriculture and Forestry Anyang 430-824 Korea department of Veterinary Internal Medicine College of Veterinary Medicine Seoul National University Seoul 151-742 Korea Genes encoding for the premembrane and envelope prME envelope E and nonstructural protein NS1 of Japanese encephalitis virus JEV were cloned. Each protein was expressed in baculovirus expression system. Of the three proteins expressed in baculovirus system only prME had hemagglutination activity. The prME 72 and 54 kDa E 54 kDa and NS1 46 kDa proteins could be detected by Western blotting in the recombinant virus infected cells. Immunogenicity of the recombinant proteins obtained from infected Spodoptera frugiperda Sf-9 cells was examined in mice. The 3 week-old ICR mice immunized intraperitoneally with three recombinant proteins three times were challenged with a lethal JEV. A survival rate was increased from about in unimmunized mice to in E prME and only E groups. The complete protection was shown in prME and live vaccine inoculated groups respectively. We also measured neutralizing antibody and three immunoglobulin subtypes of IgG1 IgG2a and IgG2b in the sera of mice before and after challenge. Titers of IgG1 antibodies were approximately two to three times higher than that of IgG2b antibodies in all the immunized groups as compared to the control group. However IgG2a antibody level somewhat increased after challenge indicating T-helper type 1 Th1 cell response. The results of this study can provide useful information for developing efficacious subunit vaccine against JEV. Key words baculovirus Japanese encephalitis virus JEV protective immunity .