tailieunhanh - Báo cáo khoa hoc:" A repetitive probe for FISH analysis of bovine interphase nuclei"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: A repetitive probe for FISH analysis of bovine interphase nuclei | Genet. Sel. Evol. 32 2000 217-225 217 INRA EDP Sciences Original article A repetitive probe for FISH analysis of bovine interphase nuclei Wafa SLIMANEa b Daniel VAIMANc Sophie GODARDc Anne VAIMANc Edmond CRIBIUc Jean-Paul RENARDa a Unite biologie du développement Institut national de la recherche agronomique 78352 Jouy-en-Josas Cedex France b Services techniques de l Union national des cooperatives d elevage et d insemination artificielle 13 rue Jouet BP 65 94703 Maisons-Alfort France c Génétique biochimique et cytogénétique Institut national de la recherche agronomique 78352 Jouy-en-Josas Cedex France Received 22 June 1999 accepted 20 December 1999 Abstract - The purpose of this study was to generate repetitive DNA sequence probes for the analysis of interphase nuclei by fluorescent in situ hybridisation FISH . Such probes are useful for the diagnosis of chromosomal abnormalities in bovine preimplanted embryos. Of the seven probes E1A E4A Ba H1A W18 W22 W5 that were generated and partially sequenced five corresponded to previously described Bos taurus repetitive DNA E1A E4A Ba W18 W5 one probe W22 shared no homology with other DNA sequences and one H1A displayed a significant homology with Rattus norvegicus mRNA for secretin receptor transmembrane domain 3. Fluorescent in situ hybridisation was performed on metaphase bovine fibroblast cells and showed that five of the seven probes hybridised most centromeres E1A E4A Ba W18 W22 one labelled the arms of all chromosomes W5 and the H1A probe was specific to three chromosomes ch14 ch20 and ch25 . Moreover FISH with H1A resulted in interpretable signals on interphase nuclei in 88 of the cases while the other probes yielded only dispersed overlapping signals. satellite DNA FISH bovine centromere Résumé Generation d une sonde bovine à sequences répétées pour 1 analyse en FISH des noyaux bovins en interphase. L objectif de cette étude est d isoler des sondes nucléiques bovines spécifiques d un faible nombre de chromosomes