tailieunhanh - Báo cáo khoa học: "Comparative efficacy of standard AGID, CCIE and competitive ELISA for detecting bluetongue virus antibodies in indigenous breeds of sheep and goats in Rajasthan, India"

Tuyển tập các báo cáo nghiên cứu khoa học quốc tế về bệnh thú y đề tài: Comparative efficacy of standard AGID, CCIE and competitive ELISA for detecting bluetongue virus antibodies in indigenous breeds of sheep and goats in Rajasthan, India | J. Vet. Sci. 2005 6 1 77-79 JOURNAL OF Short Communication Veterinary Science Comparative efficacy of standard AGID CCIE and competitive ELISA for detecting bluetongue virus antibodies in indigenous breeds of sheep and goats in Rajasthan India Smriti Shringi B. N. Shringi Department of Veterinary Microbiology College of Veterinary Science and Animal Husbandry Rajasthan Agricultural University Bikaner-334 001 Rajasthan India The sero-prevalence of antibodies against blue tongue virus BTV in 408 local breeds of sheep in Rajasthan state in India was investigated using standard agar gel immunodiffusion AGID test. Maximum seropositivities of 13 115 13 121 11 155 and 1 17 were recorded in the Chokla Magra Nali and Pugal breeds respectively. Out of 107 goat serum samples 6 were AGID positive. The performance of the standard AGID counter current immuno-electrophoresis CCIE and the competitive enzyme-linked immunosorbent assay cELISA for the detection of serum antibody against BTV in indigenous breeds of sheep were compared. Out of 178 sheep serum samples tested 17 22 and 54 were positive for group-specific bluetongue antibodies by AGID CCIE and cELISA respectively. There was appreciable difference in the seroprevalence detected by AGID CCIE and cELISA in clinically healthy and diseased sheep with regard to relative sensitivities and specificities of the tests with cELISA being highly sensitive and specific followed by CCIE and AGID test. It was concluded that these indigenous breeds of sheep may be a potential reservoir of BTV infection and cELISA should be routinely used for the detection of antibodies against BTV in these local breeds of sheep. Key words Blue tongue seroprevalence AGID CCIE competitive ELISA Bluetongue infection caused by blue tongue virus BTV belonging to family reoviridae genus Orbivirus is considered as one of the most important diseases of domestic livestock. BT exists around the world in a broad band covering much